METHODOLOGY AND METHOD OF EVALUATION OF OXIDATIVE MODIFICATION OF PROTEINS IN COMPLEX WITH AVERAGE WEIGHT MOLECULES, PROSPECTS FOR THEIR USE
Study of oxidative modification of proteins (OMP) and the average mass molecules (MSM) as markers of the severity of endotoxemia, sensitive to changes in the degree of oxidative stress in the complex, one sample of the biological environment, methodologically justified, since it will increase the information content of each of these parameters and reduce the flow of material used, which is particularly important in clinical biochemistry. OMP evaluation method in combination with MSM implemented by us in accordance with the author’s method (patent for the invention of the Russian Federation no. 2012153044, priority of 12.07.2012, the decision to grant a patent on 14.04.2014, in collaboration) and tested on a model biological system conditions spontaneous and Fe2+-induced OMP.
Keywords: oxidative modification of proteins, average mass molecules, methodology, method, model biological system
References:
1. Belonogov R. N., Titova N. M., Dykhno Yu. A., Lapeshin P. V., Kudryashov E. V., Savchenko A. A. Oxidative modifi cation of proteins and lipids of blood plasma of patients with lung cancer. Siberian Journal of Oncology, 2009, no. 4, рp. 48–51 (in Russian).
2. Kopitova T. V., Dmitrieva O. N., Himkina L. I., Panteleeva G. A. Oxidative modifi cation of proteins and oligopeptides in patients with chronic dermatoses with the syndrome of endogenous intoxication. Fundamental Research, 2009, no. 6, рp. 25–29 (in Russian).
3. Kelina N. Yu., Bezruchko N. V., Rubtsov G. K. Biochemical manifestations of endotoxemia: methodological aspects of the study and evaluation, predictive value (analytical review). Bulletin of the Tyumen State University, 2012, no. 6, рp. 143–147 (in Russian).
4. Khaldun O., Lakal A., Bekshokov K. S., Klichkhanov N. K. Oxidative modifi cation of plasma proteins of burn patients. Bulletin of Dagestan State University, 2012, no. 1, рp. 128–132 (in Russian).
5. Bezruchko N. V., Rubtsov G. K., Anopin K. D., Krivchenkova E. V. Clinical and biochemical perspectives of development of non-invasive methods for assessing endogenous intoxication, the value indicators of urine and saliva. Technology of Living Systems, 2013, no. 1, рp. 47–52 (in Russian).
6. Rubtsov G. K., Bezruchko N. V., Sadovnikova D. G., Kozlova G. A., Anopin K. D. Clinical and biochemical study of integrated average weight molecules and oxidative modifi cation of proteins for evaluation of endotoxemia. Questions of Biological, Medical and Pharmaceutical Chemistry, 2013, no. 2, рp. 41–47 (in Russian).
7. Nizkodubova S. V., Lasukova T. V., Legostin S. A. Correction of energy metabolism impairments in tetrachlormethane-poisoned rat liver tissue by means of sapropel lipids. Tomsk State Pedagogical University Bulletin, 2013, vol. 8, pp. 94–99 (in Russian).
8. Klebanov G. I., Babenkova I. V., Teselkin Yu. O, Komarov O. S., Vladimirov Yu. А. Evaluation of antioxidant activity of blood plasma using yolk lipoprotein. Laboratory Business, 1988, no. 5, рp. 59–62 (in Russian).
9. Burmistrova L. A. Physico-chemical analysis and biochemical assessment of the biological activity of drone brood. Diss.... cand. biol. Sci. Ryazan, 1999. 173 p. (in Russian).
10. Rubtsov G. K., Bezruchko N. V., Gengin M. T., Vasil’kov V. G., Borisova E. Yu., Anopin K. D., Vasilieva A. D., Sadovnikova D. G., Kozlova G. A., Kruchinina A. D., Gamzin S. S. Method for determining oxidative modifi cation of proteins in pool of average molecular weight substances in blood serum, plasma, red blood cells and urine. An application for a patent of the Russian Federation № 2012153044, priority of 07.12.2012, the decision to grant a patent on 14.04.2014 (in Russian).
11. Dubinina E. E., Burmistrov S. O., Khodov D. A., Porotov I. G. Oxidative modifi cation of proteins of human serum, the method of its determining. Issuess of Medical Chemistry, 1995, no. 1, рp. 24–26 (in Russian).
12. Glyants S. Biomedical Statistics. (Russ. ed.: Glyants S. Mediko-biologicheskaya statistika. Moscow, Praktika Publ., 1998. 459 p.).
13. Bezruchko N. V., Rubtsov G. K. Modeling biological systems and the method of assessment of spontaneous and initiated oxidative modifi cation of proteins in a pool of molecules of average weight. Technology of Living Systems, 2013, no. 5, рp. 46–50 (in Russian).
14. Rubtsov G. K. Complete application of model biological systems for assessment of oxidative modifi cation of proteins in the pool of average weight molecules. Tomsk State Pedagogical University Bulletin, 2013, vol. 8, рp. 81–90 (in Russian).
15. Rubtsov G. K., Bezruchko N. V., Gengin M. T., Cornfl ower V. G., Borisova E. Yu., Anopin K. D., Vasilieva A. D., Sadovnikova D. G., Kozlova G. A., Kruchinina A. D., Gamzin S. S. The method of analysis of oxidative modifi cation of proteins in a pool of average molecular weight substances. Clinical Laboratory Diagnostics, 2013, no. 9, рp. 93 (in Russian).
( 813.52 kB ) 
( 703.14 kB )
Issue: 8, 2014
Series of issue: Issue 8
Rubric: SCIENTIFIC REPORTS
Pages: 185 — 189
Downloads: 1529